The Florida Highway Patrol says two commercial vehicles and a personal vehicle were involved in a crash Tuesday morning on I-95 southbound at Mile Marker 326, north of World Golf Village.
At least one of the large vehicles overturned and there is a fuel leak, troopers said.
All southbound lanes of I-95 southbound are shut down and traffic is being diverted to County Road 210.
Two people were injured and at least one was flown by helicopter to a hospital.
St. Johns County deputies and St. Johns County Fire Rescue are on the scene, along with FHP, DOT and environmental specialists.
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