Aston Villa enter the top 20 after competing in Europe last season for the first time since 2011.
Nine Premier League clubs are in the top 20, with Arsenal, Liverpool, Tottenham, Chelsea, Newcastle and West Ham retaining their places.
Lyon are the only other new club, with Napoli and Eintracht Frankfurt dropping out.
A further five Premier League clubs are in the top 30, with Brighton 21st after competing in the Europa League for the first time in their history.
Crystal Palace, Everton, Fulham and Wolves are ranked 26th to 29th.
Revenues for the top 20 clubs rose by 6% to a record £9.47bn.
Matchday revenue was the fastest growing revenue stream, rising by 11% to £1.77bn, helped by an increase in stadium capacity, ticket prices and premium hospitality.
Real benefited most from an increase in matchday revenues, generating £210m – double last year’s figure – after renovation of their Bernabeu Stadium.
Barcelona dropped from fourth to sixth after a £53m fall in matchday revenue, with games played at a smaller stadium while the Nou Camp is redeveloped.
Commercial revenue remained the largest revenue source in the Money League, rising 10% to £4.14bn and accounting for 44% of total revenue, helped by the hosting of non-football live events such as concerts.
“Money League clubs continue to break records with ongoing growth in commercial and matchday revenues,” said Tim Bridge, lead partner in the Deloitte sports business group.
Total broadcast revenue remained at £3.64bn because each of the big five leagues – the Premier League, Spain’s La Liga, German Bundesliga, France’s Ligue 1 and Italy’s Serie A – are in the same domestic broadcast cycle.
*Figures converted from euros may differ from previously reported figures because of a change in currency exchange rates
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