The forward left the action during the first half of the 2-1 win at Anfield due to an issue, with Darwin Nunez replacing him after 30 minutes of play.
Jota will now be assessed by the Reds’ medical team; however, Slot is anticipating him being ruled out of the Champions League tie in Germany on Wednesday.
The head coach said: “Diogo had to be substituted and I am not sure what it is, but I’d be surprised if he is there on Wednesday.”
Meanwhile, Slot revealed Liverpool had a number of fitness concerns to contend with in the build-up to the encounter with Chelsea.
“I have a lot of confidence in this team, but you also saw today – after the international break – that we have some problems,” he explained.
“Conor Bradley is not in; Trent [Alexander-Arnold], you probably don’t know, was also not training in the first days after he came back, so he was a bit of a doubt before we started the game. [And] then we had Macca [Alexis Mac Allister] being sick.”
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